School of Science and Health - Seminar Series
- Event Name
- School of Science and Health - Seminar Series
- Date
- 7 September 2012
- Time
- 12:30 pm - 01:30 pm
- Location
- Parramatta Campus
Address (Room): Parramatta North, Building LZ.G.14
- Description
Title: Simple and robust separation of monosaccharides in complex mixtures by capillary electrophoresis and high performance liquid chromatography
When: Friday 7th September, 12.30 pm – 1.30 pm followed by refreshments
Venue: Building LZ.G.14, Parramatta North
Presenter: James Oliver
Overview: Plant fibre contains a complex mixture of carbohydrates that is partially liberated by acid hydrolysis. Hydrophilic Interaction Liquid Chromatography (HILIC), ligand-exchange liquid chromatography or other forms of LC with ion-exchange columns are popular techniques for analysis of acid hydrolysed plant samples. Capillary electrophoresis (CE) can also be used without derivatization at pH 12.6 with no sample pre-treatment other than dilution. Direct detection in CE of mono- and disaccharides is unexpectedly possible at 270 nm by photo-oxidation. This detection was investigated by NMR, CE and Photo-irradiation.
Speakers: James Oliver, PhD candidate at UWS
- Contact
-
Name: Sarah Chandler
Phone: 02 9685 9934
School / Department: School of Science and Health
