Integrative Physiology

Integrative Physiology - Computer Screen

Professor Vaughan Macefield
Senior Lecturer:  Dr David Mahns

Professor Vaughan Macefield

Prof Vaughan Macefield is Foundation Chair of Integrative Physiology at the School of Medicine, Western Sydney University, and a Conjoint Senior Principal Research Fellow at Neuroscience Research Australia. A former NHMRC Senior Research Fellow, he completed his PhD in neurophysiology at UNSW in 1986, and undertook advanced training in human neurophysiology in Sweden and the US, before establishing his own laboratories at Prince of Wales Medical Research Institute in 1994.

Vaughan Macefield

Vaughan is also the NSW Convenor of the Australian Brain Bee Challenge, Australia's only neuroscience competition for high school students, and has participated in a number of events including Science in the Suburbs to promote science to school audiences.

Professor Macefield's Research

Professor Macefield specializes in recording from single nerve fibres via tungsten microelectrodes inserted into the peripheral nerves of awake human subjects, and is known nationally and internationally as a world expert in recording the firing properties of human sympathetic neurones (e.g. those supplying blood vessels) in health and disease and as a leading investigator in human sensorimotor control. In 1996 he was awarded the $10,000 Sunderland Award for "Excellence in Sensorimotor Biology."

He has active collaborations with many groups in universities and hospitals in Australia and in Sweden and the USA, and has attracted postdoctoral scientists from Sweden, Denmark, Canada and Australia. For over 10 years Prof Macefield has been been examining the changes in control of the autonomic nervous system following human spinal cord injury. Over the last decade his research has extended into the study of pain and its effects on the autonomic and somatic nervous systems, using brain imaging techniques (fMRI) to study the processing of pain originating in muscle and skin.

His research is supported by the National Health and Medical Research Council of Australia and the Australian Research Council.

Professor Macefield's Team

  • Dr Rachael Brown, Associate Research Fellow. Rachael was formerly a clinical nurse specialist working in the acute spinal unit, and it was her interest in blood pressure control following a spinal cord injury that lead her to undertake a PhD with Professor Macefield which she received in 2009. She has since been working with Professor Macefield, utilizing the technique of microneurography, with a focus on cardiovascular control. Recently, Rachael published work showing, for the first time, that watching a 'first person' video of someone else running can increase cardiovascular parameters such as heart rate, despite the fact the subject is sitting relaxed with no muscle activity. Her current research involves examining gender differences in muscle sympathetic nerve activity during long-lasting muscle pain.
  • Dr Paul Breen, Visiting Fellow. Paul received his BEng Degree in Computer Engineering (First Class Honours) in 2003 and his PhD in 2007, both from the University of Limerick. His PhD research investigated the application of Neuromuscular Electrical Stimulation for blood flow assistance. He was awarded a Government of Ireland Postdoctoral Fellowship in 2007 from the Irish Research Council for Science, Engineering and Technology to continue this work. In 2008 he was awarded a Postgraduate Certificate in Teaching and Learning in Higher Education from the National University of Ireland, Galway. He has worked in collaborator labs at Roessingh Research & Development, Enschede, The Netherlands and Harvard Medical School, Boston, USA. His current research interest involves investigating the potential of subsensory electrical noise as a treatment for the loss of sensory function. This work, supported by a Health Research Board Award, aims to restore lost neural functionality where it is impaired through neuropathy. Currently a Visiting Fellow at the Western Sydney University, Paul is using microneurography to investigate the neural response to this treatment paradigm.
  • Dr Ingvars Birznieks, Research Fellow. Ingvars' undertook his PhD at the University of Umea, Sweden, and has been in Sydney since 2005. His research interests revolve around sensorimotor processing, tactile sense, perception, tactile sensorimotor control of the human hand, dexterous manipulation of objects, haptic devices, pain mechanisms, functional and clinical consequences of somatosensory disorders in stroke patients. One of his signature research methods is microelectrode recordings from single tactile afferents and muscle spindle afferents in awake human subjects (human microneurography). In 2011 he was appointed Senior Lecturer in Physiology at the School of Science and Health, Western Sydney University. His research network extends also to Neuroscience Research Australia, the Graduate School of Biomedical Engineering and School of Medical Sciences at The University of New South Wales, as well as the School of Health Sciences at The University of Sydney. Since 2011, as a chief investigator, Ingvars has received more than $1.4 million in competitive funding (two ARC and three NHMRC grants).
  • Dr Chloe Taylor, Lecturer. Chloe undertook her PhD at Liverpool John Moores University in the UK and has been employed as a lecturer in Sport and Exercise Science at Western Sydney University since July 2011. Chloe's research focus is cardiovascular control, in particular the responses to physiological challenges such as exercise and orthostasis, and the effects of time and day. A key area of her research involves the assessment of cardiovagal and sympathetic baroreflex sensitivity and its role in blood pressure regulation with respect to these challenges. Chloe is currently conducting a study of inter-individual differences in blood pressure responses to mental and physical stressors, looking specifically at the effects of sex and ageing. She is also involved in a project using microneurography to measure sympathetic nerve activity to contracting muscles, exploring the roles of central command and the metaboreflex. 
  • Azharuddin Fazalbhoy, PhD student. Azharuddin is a M Osteopath graduate of Western Sydney University who is currently enrolled in a PhD program through UNSW, with the experimental work being undertaken in the School of Medicine, Western Sydney University. The project aims to examine the effects of tonic muscle pain, induced by infusion of hypertonic saline into a leg muscle, on muscle and cutaneous sympathetic outflow and on the fusimotor system. This work involves microelectrode recordings from the common peroneal nerve in awake human subjects.
  • Rania Fatouleh, PhD student. Rania's work aims to identify the brain sites responsible for the increase in muscle sympathetic nerve activity (MSNA) in obstructive sleep apnoea (OSA). Increased MSNA is a precursor to hypertension and elevated cardiovascular morbidity and mortality. However, the mechanisms underlying the high MSNA in OSA are not well understood. By recording MSNA concurrently with functional Magnetic Resonance Imaging (fMRI) Rania is aiming to identify the central processes responsible for the sympathoexcitation. Long-term treatment with continuous positive airway pressure (CPAP) decreases MSNA in OSA but this is not reflected in a fall in blood pressure. Therefore Rania is testing the same OSA patients after 6 and 12 of CPAP to detect all changes after treatment. 
  • Elie Hammam, PhD student. Elie's overall interest lays in the examination of cardiovascular control. His current research involves the examination of structural changes in the brain of patients with congestive heart failure (CHF). Employing microneurography and functional magnetic resonance imaging (fMRI) of the brainstem and the whole brain with heart failure patients allows us to further explore the neurophysiology of blood pressure control in health and disease. The study involves research before and after heart transplantation. In additional studies, Elie aims to understand how the vestibular system affects cardiovascular control. Slow movements of the body, such as those associated with sway in tall buildings may lead to motion sickness in certain individuals, which is heralded by decreases in skin blood flow and increases in sweating. The sympathetic nervous system, which is responsible for control of blood pressure, skin blood flow and sweating, is assessed using the technique of microneurography whilst generating slow changes in vestibular inputs.
  • Khadigeh El Sayed, PhD student. Under Professor Vaughan Macefield's supervision, Khadigeh completed her Honours degree in 2011. Her project aimed at assessing the role of the vestibular system in cardiovascular control which, in turn, looked at how vestibular inputs from each side of the head modulate sympathetic nerve activity to muscle and skin. This was achieved by making bilateral recordings with the application of low-frequency (0.08Hz) sinusoidal Galvanic Vestibular Stimulation (sGVS) using microneurography. Currently, her PhD project involves the investigation of inter-individual differences in muscle sympathetic nerve activity (via microneurography) and blood pressure responses to mental and physical stressors in healthy young males and females. Following this, she will also look at the effects of aging on these responses.
  • Daniel Boulton, PhD student. Daniel is a Sport and Exercise Science graduate who completed Honours in 2012 and is currently undertaking a PhD degree. Daniel's research is investigating the role of central command and peripheral reflexes in the control of muscle sympathetic nerve activity to active and inactive limbs during exercise. Microneurography is used to directly record nerve activity in both resting and exercising limbs.
  • Michael Leitch, PhD student. Michael has previously shown that irregular trains of stimuli, emulating the firing of actual motoneurones during voluntary contractions, produce greater contractile responses of single motor units than regular (non-physiological) trains of identical mean frequency but zero variability. In the current research he intends to extend this line of reasoning, through a series of experiments, utilizing intraneural microstimulation of single human motor axons. Michael will attempt to create optimal patterns that maximize the contractile responses of human motor units. By using optimized patterns of stimuli that emulate the firing of real motoneurones during voluntary contractions he hopes to be able to contribute important new information on muscle physiology and develop novel means of applying functional electrical stimulation (FES) to muscles that have been weakened or paralysed by stroke or spinal cord injury.
  • James Wright, PhD student. James is attempting to build a closed loop neuroprosthetic assistive device for suffers of motor impairment. By using microneurography to record afferent sensory activity during object manipulation the project aims to incorporate natural sensory feedback as a sensor signal in the control of a neuroprosthetic.
  • Sophie Kobuch, Bachelor of Medical Research student. Sophie will be studying the effects of long-lasting muscle pain, induced by intramuscular infusion of hypertonic saline, on sympathetic outflow to muscle in awake human subjects. It has been shown that in some subjects, this form of pain causes muscle sympathetic nerve activity, blood pressure and heart rate to fall, while in others they increase. This is despite the fact that both groups of subjects rate the pain identically, and describe it identically. In order to determine whether there is a gender effect contributing to this result, a group of male and female subjects will be studied, the latter being in low-hormone (which correlates with low MSNA) and high-hormone phase (which correlates with high MSNA) of their menstrual cycle. In addition, it is unknown whether there is any difference in the psychological profiles of the two groups of subjects.  In order to assess whether there is any difference in anxiety levels, the State and Trait Anxiety Questionnaire will be filled out each session.  

Dr David Mahns

Dr David Mahns is a Senior Lecturer in Integrative Physiology at the School of Medicine, Western Sydney University. David completed his PhD in cardiovascular physiology at Prince of Wales Medical Research Institute in 1999, before undertaking postdoctoral training in neurophysiology at the University of New South Wales and being appointed as a Lecture in Physiology at the University of New South Wales in 2003. In 2006 David established his own laboratories at the Western Sydney University. 

Dr Mahns' Research

In most circumstances we can readily distinguish between painful and non-painful stimuli. It is widely appreciated that non-painful and painful sensations rely on the activation of distinct groups (or classes) of sensory nerves. Despite this common perception,  it remains unclear whether distinguishing between innocuous (non-painful) and noxious (painful) stimuli result from the activation of single class of sensory nerves, convergence of inputs arising from multiple classes or the pattern of activation within the central nervous system. The broad aim of Dr Mahn's work is to define the contribution of different nerves (and central pathways) to pain arising from deep (e.g. viscera, muscle and bone) and superficial (skin) structures. In order achieve this his team is using a range of recoding techniques, that allow recording from individual nerve fibres, and psychophysical techniques that allow the group to better understand how the nervous system detects and relays information about pain.

David is the recipient of a current NHMRC project grant, Neural Mechanism of Bone Pain and a Western Sydney University Research Grant, Peripheral encoding of forces associated with manipulation by tactile afferents. He is involved in several research projects involving collaborations with Prof Macefield and Prof John Morley (Western Sydney University), Dr Ingvars Birznieks (Neuroscience Research Australia), Dr Richard Vickery (University of New South Wales) and A/Prof Gustavo Duque and Dr Wei Li (University of Sydney).

Dr Mahns' Team

  • Dr Saad Nagi, Research Fellow. What triggers the crossover between non-painful and painful sensations is yet to be fully elucidated especially in clinical pain-states such as allodynia, i.e. pain evoked by otherwise innocuous (tactile/cold) stimuli. Saad's research interests revolve around the mechanisms underlying the most intrinsic imprints of sentience such as touch, temperature and pain. Psychophysical tools are being employed in healthy and clinical individuals to explore the interplay of these sensations, and the role of different peripheral nerve fibres in coding of stimulus features. Recent investigations have demonstrated that a class of low-threshold unmyelinated mechanoreceptors, dubbed C-tactile fibres, mediates the crossover between pleasurable-touch and painful-touch, thereby unveiling a novel substrate of tactile allodynia, or more broadly pain modulation. Further investigations are under way with the aim of determining the response properties and innervation patterns of this afferent class, in addition to exploring the role of cognitive influences in pain modulation. 
  • Sumaiya Shaikh, PhD student. Sumaiya's previous work was to determine the neural correlates of affective sensations with pain using psychophysical methods. She is also a part of the broader project testing pain symptoms behaviourally duing sutureless peripheral nerve repair and is currently involved in projects which test the anatomical changes in cutaneous sensory mediators after injuries. She is also using a range of immunohistochemical techniques and staining methods to visualise what derives the change, centrally and/or peripherally after acute and chronic nerve injuries particularly focusing on tactile and cold allodynia.
  • Mohamad Samour, PhD student. The sensory function of the peripheral and central nervous system is frequently described as following predictable or rigid rules wherein individual nerves perform specific tasks with little overlap. However, it is increasingly appreciated that sensory systems can operate in a diversity of modes in effect revealing an underlying plasticity within the sensory systems. Through human psychophysical studies and electrophysiological experiments, my goal is to understand the fundamental functions of one of the most underrepresented group of fibres, C fibres, in reference to pain, tactile sensation and thermal sensibility.
  • James Dunn, Honours student. James is a graduate of the Bachelor of Science (Advanced) program at Western Sydney University, majoring in human biosciences. The objective of his current research is to re-explore Weber's illusion, a somewhat forgotten tactile illusion that makes the body perceive cold objects as heavier than normal ones despite there being no difference in the weight of the objects. Along with the rest of the research group James will also investigate possible ways in which transcranial magnetic stimulation can impact upon pain memory and pain perception.