Doctor Gabriel Perrone

• Qualifications
• Biography
• Research
• Selected Publications
• Grants/Current Projects
• Teaching Interests

Qualifications

 PhD (Biochemistry)

Biography

Dr Gabriel Perrone is a Senior Lecturer in Human Genetics in the School of Biomedical and Health Sciences,

Dr Perrone graduated with a PhD in Biochemistry from the University of New South Wales (UNSW) in 2003, subsequent to which he has held a research fellow position at the same institution in the School of Biotechnology and Biomolecular Sciences.

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Research

The research conducted by Dr Peronne's group centres on exploiting the model organism Saccharomyces cerevisiae to investigate a range of biological questions. A main area of interest has been to determine how cells respond to stress conditions including oxidative, heat, heavy metal, acid/pH and endoplasmic reticulum stress. As part of this approach the group is studying how cells sense, set and maintain appropriate redox homeostasis in distinct cellular compartments. In a separate area of research the group is indentifying the cellular functions, and molecules, that influence aggregation of the Alzheimer’s disease associated peptide, amyloid beta. Gabriel has extensive expertise in using genome-wide chemical-genetic, genetic-genetic and biochemical analyses to identify potential mechanisms of drug/toxin action.

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Grants/Current Projects

• Responses of cells to stress conditions including oxidative, heat, heavy metal and aberrant protein homeostasis
• Maintenance of compartmental redox homeostasis
• Role of lipids in tolerance of cells to stress
• Exploiting the model eukaryotic organism Saccharomyces cerevisiae to study aspects relating to degenerative disorders
• High-throughput chemical-genetic analyses to investigate mechanism of action of drugs and toxins

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Teaching Interests

• Genetics
• Cell Biology
• Cellular Stress Responses
• Ageing
  

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Selected Publications

Ayer, A., Tan, S.X., Grant, C.M., Meyer, A.J., Dawes, I.W. and Perrone, G.G. (2010). The critical role of glutathione in maintenance of the mitochondrial genome. Free Radic Biol Med. 15, 1956-86.

Greetham, D., Vickerstaff, J., Shenton, D., Perrone, G.G., Dawes, I.W., and Grant, C.M. (2010). Thioredoxins function as deglutathionylase enzymes in the yeast Saccharomyces cerevisiae. BMC Biochem. 11, 3.

Tan, S.X., Greetham, D., Raeth, S., Grant, C.M., Dawes, I.W. and Perrone, G.G. (2010). The thioredoxin-thioredoxin reductase system can function in vivo as an alternative system to reduce oxidized glutathione in Saccharomyces cerevisiae. J. Biol. Chem. 285, 6118-26.

*Thorsen, M., *Perrone, G.G., Kristiansson, E., Traini, M., Dawes, I.W., Nerman, O., and Tamas, M.J. (2009). Genetic basis of arsenite and cadmium tolerance in Saccharomyces cerevisiae. BMC Genomics 10, 105 *Thorsen and Perrone contributed equally.

Tan, S.X., Mariati, L.M., Dawes, I.W. and Perrone, G.G. (2009). Cu, Zn Superoxide dismutase and NADP(H) are required for tolerance of endoplasmic reticulum stress in Saccharomyces cerevisiae. Mol. Biol. Cell. 20 (5), 1493-1508.

Herst, P.M., Perrone, G.G., Dawes, I.W., Bircham, P. and Berridge, M.V. (2008). Plasma membrane electron transport in Saccharomyces cerevisiae depends on the presence of mitochondrial respiratory subunits FEMS Yeast Res., 8 (6):897-905.

Perrone, G.G., Tan, S., and Dawes, I.W. (2008). BBA-Mol.  Reactive oxygen species and yeast apoptosis. Cell Res., 1783 (7), 1354-1368.

Ng, C.H., Tan, S.X., Perrone, G.G., Thorpe, G.W., Higgins, V.J. and Dawes, I.W. (2008). Adaptation to hydrogen peroxide in Saccharomyces cerevisiae: the role of NADPH-generating systems and the SKN7 transcription factor. Free Radic. Biol. Med. 44 (6), 1131-45.

*Dilda, P.J., *Perrone, G.G., Philp, A., Lock, R.B., Dawes, I.W., and Hogg, P.J. (2008). Insight into the selectivity of arsenic trioxide for acute promyelocytic leukemia cells by characterizing Saccharomyce cerevisiae strains that are sensitive or resistant to the metaloid. Int. J. Biochem. Cell Biol. 40 (5), 1016-1029. *Dilda and Perrone contributed equally.

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