Doctor Patrice Castignolles

LECTURER,
Science (SoSH)

Personal
Contact
Biography
Publications
Research
Supervision

Personal

UWS Organisational Unit (School / Division)

Science (SoSH)

Professional Memberships

French Polymer Group GFP (2001-06-03)

Contact

Email:	P.Castignolles@uws.edu.au
Extension:	9970
Mobile:	0413021562
Location:	LZ.G.82 Parramatta
Website:

Biography

I have made significant contributions to the understanding of polymerization kinetics and the separation and characterization of polymers, especially branched polymers, polyelectrolytes and polysaccharides.

In terms of polymerization kinetics, using a combination of theory, simulation and experiment, I showed that certain reactions (viz., transfer) with dead polymer chains play a major role in the (radical) polymerization of alkyl acrylates, widely used industrially (in adhesives, coatings and paints). It was published in several seminal papers, including one on benchmark rate coefficients, under the auspices of IUPAC (the interntional governing body in chemistry).

I have made important contributions to the understanding of the separation of polymers by liquid chromatography (particularly size-exclusion chromatography, SEC, also knonw as GPC). I showed that SEC is generally applied in a limited and too often also incorrect way. I showed how these problems could be corrected by proper use of apparent molar masses (e.g. polystyrene -equivalent or pullulan-equivalent molar masses) determined by SEC in controlled/living polymerization or for the determination of transfer coefficients in radical polymerization. I derived the first rigorous expression for the signals of multiple detectors in SEC, and showed how such data can be processed correctly for complex polymers. I have applied this theory to the first accurate characterization of branched poly(alkyl acrylates), branched glucans and amphiphilic and double-hydrophilic block copolymers (pH-responsive and thermoresponsive) , studies which give considerable insight into the (bio)syntheses of these important classes of polymers. I showed that the local dispersity can be determined using multiple-detection SEC and used to estimate the accuracy of the molecular weight determined by SEC. In the cases where the accuracy of the moleclar weight is low (e.g. 100 % error), I have shown that hydrodynamic volume distribution can lead to meaningful and accurate information.

I have proposed free solution capillary electrophoresis as an alternative to liquid chromatography in the critical conditions for the separation and characterization of polyelectrolytes and polysaccharides by their structure such as composition (copolymer) or branching. The method, capillary electorphoresis in the critical condtions, can be used even to characterize soluble fractions in suspensions (no flitration needed) as in the case of gellan gums. it can separate conjugates according tothe functionalization for example chitosan conjugates.

I graduated in Paris, University Pierre and Marie Curie under Prof Jean-Pierre Varion and Prof Bernadette Charleux in 2003. My first post-doc was in the Max Planck Institute for Polymer Reserach, Minaz, in 2004-2005. I was then research Fellows in the Key Centre for Polymer (and) Colloids (KCPC) in the University of Sydney (2005-2006), the Centre for Nutrition and Food Sciences (CNAFS) in the University of Queensland (2006-2008) and the Insitute of Physical Chemistry at the University of Mainz (2009). In 2010, I joined UWS and the Australian Centre for Research on Separation Science (ACROSS).

This information has been contributed by Doctor Castignolles.

Committees

SoSH research seminars on Parramatta campus

Publications

Journal Articles

Lefay, C., Gaborieau, M., Bertin, D., Gigmes, D., Guillaneuf, Y., Moreira, G., Thevarajah, J., Castignolles, P., Ziarelli, F., Bloch, E., Major, M. and Charles, L. (2013), 'Heterogeneous modification of chitosan via nitroxide-mediated polymerization', Polymer Chemistry, 7.
Delaittre, G., Save, M., Gaborieau, M., Castignolles, P., Rieger, J. and Charleux, B. (2012), 'Synthesis by Nitroxide-Mediated Aqueous Dispersion Polymerization, Characterization, and Physical Core-Crosslinking of pH- and Thermoresponsive Dynamic Diblock Copolymer Micelles', Polymer Chemistry, 13.
Taylor, D., Ferris, C., Maniego, A., Castignolles, P., In Het Panhuis, M. and Gaborieau, M. (2012), 'Characterization of Gellan Gum by Capillary Electrophoresis', Australian Journal of Chemistry, 9.
Castignolles, P. (2012), 'Williams, S. Kim R.; Caldwell, Karin D. (Eds.): Field-flow fractionation in biopolymer analysis', Analytical and Bioanalytical Chemistry, 2.
Taylor, D., Ferris, C., Maniego, A., Castignolles, P., Panhuis, M. and Gaborieau, M. (2012), 'Characterization of gellan gum by capillary electrophoresis', Australian Journal of Chemistry, 9.
Delaittre, G., Save, M., Gaborieau, M., Castignolles, P., Rieger, J. and Charleux, B. (2012), 'Synthesis by nitroxide-mediated aqueous dispersion polymerization, characterization, and physical core-crosslinking of pH- and thermoresponsive dynamic diblock copolymer micelles', Polymer Chemistry, 13.
Colombani, O., Langelier, O., Martwong, E. and Castignolles, P. (2011), 'Polymerization kinetics: monitoring monomer conversion using an internal standard - key Role of sample t0', Journal of Chemical Education, 6.
Gaborieau, M. and Castignolles, P. (2011), 'Size-exclusion chromatography (SEC) of branched polymers and polysaccharides', Analytical and Bioanalytical Chemistry, 11.
Gaborieau, M., Causon, T., Guillaneuf, Y., Hilder, E. and Castignolles, P. (2010), 'Molecular weight and tacticity of oligoacrylates by capillary electrophoresis - mass spectrometry', Australian Journal of Chemistry, 8.
Gaborieau, M., Koo, S., Castignolles, P., Junkers, T. and Barner-Kowollik, C. (2010), 'Reducing the degree of branching in polyacrylates via midchain radical patching: a quantitative melt-state NMR study', Macromolecules, 4.
Castignolles, P. and Gaborieau, M. (2010), 'Viscosimetric detection in size-exclusion chromatography (SEC/GPC): The Goldwasser method and beyond', Journal of Separation Science, 7.
Junkers, T., Schneider-Baumann, M., Koo, S., Castignolles, P. and Barner-Kowollik, C. (2010), 'Determination of Propagation Rate Coefficients for Methyl and 2-Ethylhexyl Acrylate via High Frequency PLP-SEC under Consideration of the Impact of Chain Branching', Macromolecules, 8.
Schmitz, S., Dona, A., Castignolles, P., Gilbert, R. and Gaborieau, M. (2009), 'Assessment of the extent of starch dissolution in dimethylsulfoxide by 1H NMR spectroscopy', Macromolecular Bioscience, 9.
Gaborieau, M., De Bruyn, H., Mange, S., Castignolles, P., Brockmeyer, A. and Gilbert, R. (2009), 'Synthesis and characterization of synthetic polymer colloids colloidally stabilized by cationized starch oligomers', Journal of Polymer Science A: Polymer Chemistry, 17.
David, G., El Asri, Z., Rich, S., Castignolles, P., Guillaneuf, Y., Lacroix-Desmazes, P. and Boutevin, B. (2009), 'Peculiar behavior of degenerative chain transfer polymerization of a phosphonated methacrylate', Macromolecular Chemistry and Physics, 9.
Castignolles, P., Graf, R., Parkinson, M., Wilhelm, M. and Gaborieau, M. (2009), 'Detection and quantification of branching in polyacrylates by size-exclusion chromatography (SEC) and melt-state 13C NMR spectroscopy', Polymer, 11.
Castignolles, P. and Gaborieau, M. (2009), 'Caracterisation de l'amidon et de ses materiaux composites', Annales des Falsifications, de l'expertise chimique & toxicologique, 9.
Bar-Nes, G., Hall, R., Sharma, V., Gaborieau, M., Lucas, D., Castignolles, P. and Gilbert, R. (2009), 'Controlled/living radical polymerization of isoprene and butadiene in emulsion', European Polymer Journal, 15.
Castignolles, P. (2009), 'Transfer to polymer and long-chain branching in PLP-SEC of acrylates', Macromolecular Rapid Communications, 7.
Guillaneuf, Y. and Castignolles, P. (2008), 'Using apparent molecular weight from SEC in controlled /living polymerization and kinetics of polymerization', Journal of Polymer Science, Part A: Polymer Chemistry, 15.
Aungsupravate, O., Lucas, D., Abu Hassan, N., Tonge, M., Castignolles, P., Gaborieau, M., Warrender, G. and Gilbert, R. (2008), 'Water vapour transmission in butadiene-MMA-methacrylic acid latex films', European Polymer Journal, 15.
Gaborieau, M., Nicolas, J., Save, M., Charleux, B., Vairon, J., Gilbert, R. and Castignolles, P. (2008), 'Separation of complex branched polymers by size-exclusion chromatography (SEC) probed with multiple detection', Journal of Chromatography A, 9.
Hoang, N., Guillaneuf, Y., Castignolles, P., Landolfi, A., Kravchuk, A., Girard, E., Peate, J., Hernandez, J., Gaborieau, M., Kravchuk, O. and Gilbert, R. (2008), 'Toward a full characterization of native starch: separation and detection by size-exclusion chromatography', Journal of Chromatography A, 11.
Hernandez, J., Gaborieau, M., Castignolles, P., Gidley, M., Myers, A. and Gilbert, R. (2008), 'Mechanistic investigation of a starch-branching enzyme using hydrodynamic volume SEC analysis', Biomacromolecules, 12.
Nejad, E., Castignolles, P., Gilbert, R. and Guillaneuf, Y. (2008), 'Synthesis of methacrylate derivatives oligomers by dithiobenzoate-RAFT- mediated polymerization', Journal of Polymer Science, Part A: Polymer Chemistry, 13.
Gaborieau, M., Hernandez, J., Gilbert, R., Gray-Weale, A. and Castignolles, P. (2007), 'Theory of multiple detection of size exclusion chromatography of complex branched polymers', Macromolecular Theory and Simulations, 16.
Demir, M., Castignolles, P., Abkey, U. and Wegner, G. (2007), 'In-situ bulk polymerization of dilute particle/MMA dispersion', Macromolecules, 9.
Mange, S., Dever, C., De Bruyn, H., Gaborieau, M., Castignolles, P. and Gilbert, R. (2007), 'Grafting of oligosaccharides onto synthetic polymer colloids', Biomacromolecules, 8.
Dona, A., Yuen, W., Peate, J., Gilbert, R., Castignolles, P. and Gaborieau, M. (2007), 'A new NMR method for directly monitoring and quantifying the dissolution kinetics of starch in DMSO', Carbohydrate Research, 7.
Konkolewicz, D., Taylor, J., Castignolles, P., Gray-Weale, A. and Gilbert, R. (2007), 'Toward a more general solution to the band-broadening problem in size separation of polymers', Macromolecules, 11.
Castignolles, P., Nikitin, A., Couvreur, L., Mouraret, G., Charleux, B. and Vairon, J. (2006), 'Pulsed laser polymerization of alkyl acrylates: Potential effects of the oxygen presence and high laser power', Macromolecular Chemistry and Physics, 9.
Castignolles, P., Gaborieau, M., Hilder, E., Sprong, E., Ferguson, C. and Gilbert, R. (2006), 'High-resolution separation of oligo(acrylic acid) by capillary zone electrophoresis', Macromolecular Rapid Communications, 5.
Demir, M., Memesa, M., Castignolles, P. and Wegner, G. (2006), 'PMMA/zinc oxide nanocomposites prepared by in-situ bulk polymerization', Macromolecular Rapid Communications, 8.
Guillaneuf, Y., Bertin, D., Castignolles, P. and Charleux, B. (2005), 'New experimental procedure to determine the recombination rate constants between nitroxides and macroradicals', Macromolecules, 9.
Asua, J., Beuermann, S., Buback, M., Castignolles, P., Charleux, B., Gilbert, R., Hutchinson, R. and Leiza, J. (2004), 'Critically evaluated rate coefficients for free-radical polymerization, 5, Propagation rate coefficient for butyl acrylate', Macromolecular Chemistry Physics, 10.
Nikitin, A., Castignolles, P., Charleux, B. and Vairon, J. (2003), 'Simulation of molecular weight distributions obtained by pulsed laser polymerization (PLP). New analytical expressions including intramolecular transfer to polymer,', Macromolecular Theory and Simulations, 9.
Nikitin, A., Castignolles, P., Charleux, B. and Vairon, J. (2003), 'Determination of propagation rate coefficients of acrylates by pulsed-laser polymerization in the presence of intramolecular transfer to polymer', Macromolecular Rapid Communications, 5.
Couvreur, L., Piteau, G., Castignolles, P., Tonge, M., Coutin, B., Charleux, B. and Vairon, J. (2001), 'Pulsed-laser Radical Polymerization and Propagation Kinetic Parameters of some Alkyl Acrylates', Macromolecular Symposia, 11.

Conference Papers

De Bruyn, H., Sprong, E., David, G., Mange, S., Castignolles, P., Gaborieau, M. and Gilbert, R. (2007), 'Copolymers of starch and synthetic monomers', RACI cereal chemistry conference, .

Research

My reseach aims at establishing the relations between the structure of polymers and their synthesis (radical polymerization or biosynthesis) and the structure of polymers and their properties (such cell adhesion, drug binding, drug release etc.). We are developping new separation and characterization methods mainly based on capillary electrophoresis and SEC (as well as solid-state NMR and others).

Together with Dr Marion Gaborieau, Prof Janice Aldrich-Wright and Dr Mark Jones (UWS) and Dr Yohann Guillaneuf (Aix-Marseille University), we are developping new delivery agents for anticancer drugs. We use smart polymers synthesized in Marseille as agents. We characterize these and their binding to anticancer drugs using capillary electrophoresis in the critical conditions (and other method including solid-state NMR). Alison Maniego complete a first class honours project on this topic and is going further on a PhD in 2013.

Together with Dr Marion Gaborieau and Dr Michael O'Connor, we are developping new substrates for stem cell culture. The synthesis of the substrate is monitored (and optimized) using capillary electrorphoresis. The substrates are then characterized by solid-state NMR as well as Scanning Electron Microscopy in collaboration with Dr Ric Wuehrer.

Together with James Oliver, Dr Marion Gaborieau , Dr Michael Philips, Julie Markham and Dr Paul Peiris (UWS) and Prof Emiliy Hilder (ACROSS, University of Tasmania), we are developping fast and accurate method to determine sugar compostion in plant fiber or in bioethanol production, comparing capillary electrophoresis and the different possible forms of liquid chromatography (including ion-exchange and HILIC).

Together with Dr Marion Gaborieau, Dr Valentina Naumovski and Prof Kelvin Chan, we are investigating the relation between the structure of glucomans and the health benefit related to their consumption as food.

Together with Dr Marion Gaborieau (UWS), Dr Aurelia Charlot and Prof Etienne Fleury (INSA, Lyon, France), we characterize polysaccharides and their composities for bioplastics.

Together with Dr Marion Gaborieau (UWS) and Prof Mathias Destarac (University Paul Sabatier, Toulouse, France), we characterize new smart block copolymers.